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KMID : 0613820090190040442
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Journal of Life Science
2009 Volume.19 No. 4 p.442 ~ p.448
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Photochemical Damage and Responses of Antioxidant Enzymes in Rice Leaves Induced to Light-Chilling
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Koo Jeung-Suk
Choo Yeon-Sik
Lee Chin-Bum
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Abstract
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We investigated photooxidation and responses of antioxidant enzymes involved in scavenging reactive oxygen species (ROS) after light-chilling (4℃) for 2 days and post chilling (25℃) in rice leaves. Chilling leaves indicated a 50% reduction in photosynthetic efficiency (Fv/Fm ratio) and a 48% increase of H₂O₂, respectively, compared to the control group. In comparison with the control, activities of superoxide dismutase (SOD) and glutathione reductase (GR) increased at light-chilling and post-chilling. CuZn-SOD and Mn-SOD among SOD forms were detected in rice leaves, while Fe-SOD was not found. The increase of SOD and GR activity may serve as a basis for defense against chilling injury as it dismutase superoxide generated by light-chilling. Catalase (CAT) activity decreased during light-chilling, while activity of APX showed remarkable increase during light-chilling in rice leaves. Among CAT isoforms analyzed by 10% native PAGE, activities of isoform -2 and -3 were inhibited during light-chilling. From the elevated APX activity and decreased CAT activity, we suggest that these two enzymes show mutual supplementary relationships, indicating different tendency during light-chilling.
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KEYWORD
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Chilling, H₂O₂, Fv/Fm, rice, ROS scavenging enzymes
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